Improved immunocytochemical staining through the use of Fab fragments of primary antibody, Fab-specific second antibody, and Fab-horseradish peroxidase.
نویسندگان
چکیده
منابع مشابه
Use of digoxin-specific antibody fragments (Fab) in the management of digoxin poisoning.
Massive digoxin overdosage is an uncommon but serious medical emergency with a mortality of 20%.1 It involves multiple management problems including hyperkalaemia, heart block and resistant ventricular arrhythmias. The management of the problems associated with severe digoxin overdosage has been assisted by two recent therapeutic advances, the use of digoxin-specific antibody fragments (Fab) to...
متن کاملPreparation of recombinant human monoclonal antibody Fab fragments specific for Entamoeba histolytica.
Genes coding for human antibody Fab fragments specific for Entamoeba histolytica were cloned and expressed in Escherichia coli. Lymphocytes were separated from the peripheral blood of a patient with an amebic liver abscess. Poly(A)+ RNA was isolated from the lymphocytes, and then genes coding for the light chain and Fd region of the heavy chain were amplified by a reverse transcriptase PCR. The...
متن کاملDifferent mechanisms of antibody-mediated neutralization of parvoviruses revealed using the Fab fragments of monoclonal antibodies.
Antibody binding and neutralization are major host defenses against viruses, yet the mechanisms are often not well understood. Eight monoclonal antibodies and their Fab fragments were tested for neutralization of canine parvovirus and feline panleukopenia virus. All IgGs neutralized >85% of virus infectivity. Two Fabs neutralized when present at 5 nM, while the others gave little or no neutrali...
متن کاملStructure of the recombinant antibody Fab fragment f3p4.
The structure of the antibody Fab fragment f3p4, which was selected from a subset of the synthetic HuCAL antibody library to bind the sodium citrate symporter CitS, is described at 1.92 A resolution. Comparison with computational models revealed deviations in a few framework positions and in the binding loops. The crystals belong to space group P2(1)2(1)2 and contain four molecules in the asymm...
متن کاملPreparation, characterization and crystallization of an antibody Fab fragment that recognizes RNA. Crystal structures of native Fab and three Fab-mononucleotide complexes.
Fab fragments from Jel 103, an antibody which specifically binds to single-stranded poly(rl), were prepared by papain digestion, separated into eight isoforms and characterized by mass spectrometry. One of the purified isoforms yielded crystals suitable for structural studies by X-ray diffraction and its crystal structure was determined to 2.4 A resolution. Soaking the crystals in solutions con...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1985
ISSN: 0022-1554,1551-5044
DOI: 10.1177/33.7.2409131